REPLACE™ (SFSB) SOP
Standard Operating Procedures for Sentinel Free Soiled Bedding (SFSB).
Sentinel Free Soiled Bedding (SFSB) Institutional SOP
A Customizable PDF That Can Be Modified To Align With Standard Operating Procedures for Direct Colony Sampling.
Tecniplast Interceptor EDT SOP
Standard Operating Procedures for the Tecniplast Interecptor EDT.
Direct Colony Sampling SOP
Standard Operating Procedures for Direct Colony Sampling.
Allentown Sentinel EDT SOP
Standard Operating Procedures for Allentown Sentinel EDT.
Sample Collection Kit Request
Please click the button below to go to our Animal Health Monitoring request sample collection materials form, where you can request Opti-Spot® cards, swabs, tubes, or REPLACE® matrices. Requested supplies will ship within 4-7 business days via FedEx. In order to help clients manage resources, we suggest ordering supplies to cover 3 months’ worth of testing.
If you have questions regarding order status, tracking, or changes to your order, please contact us at bioanalytics-lss@idexx.com. For all other inquiries, please contact idexxbioanalytics@idexx.com.
NHP Sample Collection Kit Request
Please click the button below to go to our NHP request sample collection material form. We suggest ordering supplies to cover 3 months’ worth of testing. If you have questions about quantities or would like assistance determining items and quantities, please contact us: idexxbioanalytics@idexx.com
Animal Health Monitoring Online Submission Guide
A quick reference guide to Animal Health Monitoring sample submissions in our online portal.
There have been an increasing number of publications on environmental health monitoring in our field. Over 20 peer-reviewed publications support the use of environmental monitoring. They find that environmental health monitoring successfully detects common pathogens of concern. In fact, detection with environmental health monitoring is generally found to be as good or better than with live sentinel rodents. Further environmental health monitoring can cost less and decrease staff emotional burden.
List of Publications
- Bauer, B. A., Besch-Williford, C., Livingston, R. S., Crim, M. J., Riley, L. K., & Myles, M. H. (2016). Influence of rack design and disease prevalence on detection of rodent pathogens in exhaust debris samples from individually ventilated caging systems. Journal of the American Association for Laboratory Animal Science, 55(6), 782-788.
- Besselsen, D. G., Wagner, A. M., & Loganbill, J. K. (2000). Effect of mouse strain and age on detection of mouse parvovirus 1 by use of serologic testing and polymerase chain reaction analysis. Comparative medicine, 50(5), 498-502.
- Brielmeier, M., Mahabir, E., Needham, J. R., Lengger, C., Wilhelm, P., & Schmidt, J. (2006). Microbiological monitoring of laboratory mice and biocontainment in individually ventilated cages: a field study. Laboratory animals, 40(3), 247-260.
- Clancy, B. M., Theriault, B. R., Schoenberger, J. M., Bowers, C. J., Mitchell, C. M., Langan, G. P., Ostdiek, A.M.,, & Luchins, K. R. (2022). Identification and Control of an Ornithonyssus bacoti Infestation in a Rodent Vivarium by Using Molecular Diagnostic Techniques. Comparative Medicine.
- Compton, S. R., Homberger, F. R., Paturzo, F. X., & Clark, J. M. (2004). Efficacy of three microbiological monitoring methods in a ventilated cage rack. Comparative medicine, 54(4), 382-392.
- de Bruin, W. C. C., van de Ven, E. M. E., & Hooijmans, C. R. (2016). Efficacy of soiled bedding transfer for transmission of mouse and rat infections to sentinels: a systematic review. PloS one, 11(8), e0158410.
- Dole, V. S., Zaias, J., Kyricopoulos-Cleasby, D. M., Banu, L. A., Waterman, L. L., Sanders, K., & Henderson, K. S. (2011). Comparison of traditional and PCR methods during screening for and confirmation of Aspiculuris tetraptera in a mouse facility. Journal of the American Association for Laboratory Animal Science, 50(6), 904-909.
- Dubelko, A. R., Zuwannin, M., McIntee, S. C., Livingston, R. S., & Foley, P. L. (2018). PCR Testing of Filter Material from IVC Lids for Microbial Monitoring of Mouse Colonies. Journal of the American Association for Laboratory Animal Science, 57(5), 477-482.
- Gerwin, P. M., Ricart Arbona, R. J., Riedel, E. R., Henderson, K. S., & Lipman, N. S. (2017). PCR testing of IVC filter tops as a method for detecting murine pinworms and fur mites. Journal of the American Association for Laboratory Animal Science, 56(6), 752-761.
- Hanson, W. H., Taylor, K., & Taylor, D. K. (2021). PCR Testing of Media Placed in Soiled Bedding as a Method for Mouse Colony Health Surveillance. Journal of the American Association for Laboratory Animal Science: JAALAS.
- Ike, F., Bourgade, F., Ohsawa, K., Sato, H., Morikawa, S., Saijo, M., … & Berard, M. (2007). Lymphocytic choriomeningitis infection undetected by dirty-bedding sentinel monitoring and revealed after embryo transfer of an inbred strain derived from wild mice. Comparative medicine, 57(3), 272-281.
- Jensen, E. S., Allen, K. P., Henderson, K. S., Szabo, A., & Thulin, J. D. (2013). PCR testing of a ventilated caging system to detect murine fur mites. Journal of the American Association for Laboratory Animal Science, 52(1), 28-33.
- Kapoor, P., Hayes, Y. O., Jarrell, L. T., Bellinger, D. A., Thomas, R. D., Lawson, G. W., … & Nielsen, J. N. (2017). Evaluation of anthelmintic resistance and exhaust air dust PCR as a diagnostic tool in mice enzootically infected with Aspiculuris tetraptera. Journal of the American Association for Laboratory Animal Science, 56(3), 273-289.
- Körner, C., Miller, M., & Brielmeier, M. (2019). Detection of Murine Astrovirus and Myocoptes musculinus in individually ventilated caging systems: Investigations to expose suitable detection methods for routine hygienic monitoring. PloS one, 14(8), e0221118.
- Leblanc, M., Berry, K., Graciano, S., Becker, B., & Reuter, J. D. (2014). False-positive results after environmental pinworm PCR testing due to rhabditid nematodes in corncob bedding. Journal of the American Association for Laboratory Animal Science, 53(6), 717-724.
- Lindstrom, K. E., Carbone, L. G., Kellar, D. E., Mayorga, M. S., & Wilkerson, J. D. (2011). Soiled bedding sentinels for the detection of fur mites in mice. Journal of the American Association for Laboratory Animal Science, 50(1), 54-60.
- Luchins, K. R., Bowers, C. J., Mailhiot, D., Theriault, B. R., & Langan, G. P. (2020). Cost Comparison of Rodent Soiled Bedding Sentinel and Exhaust Air Dust Health-Monitoring Programs. Journal of the American Association for Laboratory Animal Science, 59(5), 508-511.
- Mahabir, E., Durand, S., Henderson, K. S., & Hardy, P. (2019). Comparison of two prevalent individually ventilated caging systems for detection of murine infectious agents via exhaust air particles. Laboratory animals, 53(1), 84-88.
- Mailhiot, D., Ostdiek, A. M., Luchins, K. R., Bowers, C. J., Theriault, B. R., & Langan, G. P. (2020). Comparing mouse health monitoring between soiled-bedding sentinel and exhaust air dust surveillance programs. Journal of the American Association for Laboratory Animal Science, 59(1), 58-66.
- Manuel, C. A., Pugazhenthi, U., & Leszczynski, J. K. (2016). Surveillance of a ventilated rack system for Corynebacterium bovis by sampling exhaust-air manifolds. Journal of the American Association for Laboratory Animal Science, 55(1), 58-65.
- Manuel, C. A., Pugazhenthi, U., Spiegel, S. P., & Leszczynski, J. K. (2017). Detection and elimination of Corynebacterium bovis from barrier rooms by using an environmental sampling surveillance program. Journal of the American Association for Laboratory Animal Science, 56(2), 202-209.
- Miller, M., Ritter, B., Zorn, J., & Brielmeier, M. (2016). Exhaust air dust monitoring is superior to soiled bedding sentinels for the detection of Pasteurella pneumotropica in individually ventilated cage systems. Journal of the American Association for Laboratory Animal Science, 55(6), 775-781.
- Miller, M., Ritter, B., Zorn, J., & Brielmeier, M. (2016). Exhaust air particle PCR detects Helicobacter hepaticus infections at low prevalence. J Vet Sci Technol, 7(343), 2.
- Miller, M., & Brielmeier, M. (2018). Environmental samples make soiled bedding sentinels dispensable for hygienic monitoring of IVC-reared mouse colonies. Laboratory animals, 52(3), 233-239.
- O’Connell, K. A., Tigyi, G. J., Livingston, R. S., Johnson, D. L., & Hamilton, D. J. (2021). Evaluation of In-cage Filter Paper as a Replacement for Sentinel Mice in the Detection of Murine Pathogens. Journal of the American Association for Laboratory Animal Science.
- Pettan-Brewer, C., Trost, R. J., Maggio-Price, L., Seamons, A., & Dowling, S. C. (2020). Adoption of Exhaust Air Dust Testing in SPF Rodent Facilities. Journal of the American Association for Laboratory Animal Science, 59(2), 156-162.
- Perdue, K. A., Copeland, M. K., Karjala, Z., Cheng, L. I., Ward, J. M., & Elkins, W. R. (2008). Suboptimal ability of dirty-bedding sentinels to detect Spironucleus muris in a colony of mice with genetic manipulations of the adaptive immune system. Journal of the American Association for Laboratory Animal Science, 47(5), 10-17.
- Ragland, N. H., Miedel, E. L., & Engelman, R. W. (2019). PCR prevalence of murine opportunistic microbes and their mitigation by using vaporized hydrogen peroxide. Journal of the American Association for Laboratory Animal Science, 58(2), 208-215.
- Tierce, R. K., Winn, A. A., Albers, T. M., Poueymirou, W. T., Levee, E. M., Woods, S. E., & Reddyjarugu, B. (2022). Detection and Transmission of Proteus mirabilis in Immunodeficient Mice. Journal of the American Association for Laboratory Animal Science : JAALAS, 61(3), 256–269. https://doi.org/10.30802/AALAS-JAALAS-21-000104
- Varela, M., Bibay, J., Ogden, B. E., Crim, M. J., & Htoon, H. M. (2022). Using Sterile Flocked Swabs as an Alternative Method for Rodent Health Monitoring. Journal of the American Association for Laboratory Animal Science : JAALAS, 61(4), 370–380. https://doi.org/10.30802/AALAS-JAALAS-22-000024
- Winn, C. B., Rogers, R. N., Keenan, R. A., Gerwin, P. M., Matthews, K. A., Ramirez, J. A., … & Henderson, K. S. (2022). Using Filter Media and Soiled Bedding in Disposable Individually Ventilated Cages as a Refinement to Specific Pathogen-free Mouse Health Monitoring Programs. Journal of the American Association for Laboratory Animal Science, 61(4), 361-369.
- Zorn, J., Ritter, B., Miller, M., Kraus, M., Northrup, E., & Brielmeier, M. (2017). Murine norovirus detection in the exhaust air of IVCs is more sensitive than serological analysis of soiled bedding sentinels. Laboratory animals, 51(3), 301-310.