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      IDEXX BioAnalytics now provides high quality LC-MS/MS bioanalysis 
      for small molecule compounds. In addition to standard protein 
      precipitation, IDEXX BioAnalytics offers solid phase extraction and 
      solid supported liquid extraction.

      We offer the same high-quality, flexible, and fast turnaround in our bioanalysis laboratory as we do for our current clinical and anatomic pathology services.

      Analytical Equipment

       

      • Sciex 5500+ Triple Quadrupole Mass Spectrometer
          – ESI source and APCI source
      • Shimadzu Nexera X2 HPLC
      • Integra Biosciences VIAFLO96 pipetting robot
      • Beckman Avanti JXN-26 refrigerated centrifuge
      • Mettler XPR36DR analytical balance
      • Biotage TurboVap96 evaporator
      • WinNonlin Phoenix software

      Extraction Types

       

      • Protein precipitation
          – Organic and acidic methods
      • Solid Phase Extraction
      • Solid Supported Liquid Extraction

      Sample Types We Work With

      • Blood

      • Serum

      • Urine

      • Tissue homogenates

      Bioanalytical Services: Small molecule LC-MS/MS Services

      Method Feasability

      • Detecting the compound in the mass spectrometer
          – Compound infusion
          – Ionization parameter optimization
      • Eluting the retained compound from the UHPLC column
          – Column and mobile phase selection
          – Compound injection for retention time 
             and peak shape assessment

      Development and Pre-qualification
      • Optimizing the LC-MS/MS method
      • Accuracy
          – 3 concentrations tested
      • Precision
          – 3 concentrations tested
          – Quality control samples every 10 samples
      • Linearity
          – 8 concentrations tested
      • Sensitivity
          – Limit of Detection

      Method Qualification

      Sensitivity and Linearity

      • Assessment of duplicate extracted and non-extracted 
        standard curves for linearity and sensitivity and 
        reproducibility on two or three different injection days

      Quality Controls

      • High, medium, and low concentrations
        in duplicate

      Non-GLP Sample Analysis

      • Pre- and post-analysis standard curves for QC
      • Interspersed QCs through samples
          – 10% of total number of samples tested
      • Summary report in Excel format
          – System suitability
          – Standards & QC: Reporting of used/excluded, 
             nominal, back calculated, accuracy, coefficient 
             of variation
          – Carryover
          – Samples: Calculated concentration in tabular 
             format, graphical format optional

      Fast, accurate, and reliable results from the
      team you know and trust.

      Connect with IDEXX BioAnalytics today.

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